Ontology highlight
ABSTRACT:
SUBMITTER: Hamasaki R
PROVIDER: S-EPMC2749904 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Hamasaki Ryoichi R Kato Hirotomo H Terayama Yoshimi Y Iwata Hiroyuki H Valenzuela Jesus G JG
Journal of insect physiology 20090811 11
Two transcripts coding for proteins homologous to apyrases were identified by massive sequencing of a Phlebotomus (P.) duboscqi salivary gland cDNA library. The sequence analysis revealed that the amino acids important for enzymatic activity including nucleotidase activity and the binding of calcium and nucleotides were well conserved in these molecules. A recombinant P. duboscqi salivary apyrase was expressed in Escherichia coli and purified. The resulting protein efficiently hydrolyzed ADP and ...[more]