Ontology highlight
ABSTRACT:
SUBMITTER: Chumachenko NV
PROVIDER: S-EPMC2750092 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Chumachenko N V NV Novikov Y Y Yarus M M
Journal of the American Chemical Society 20090401 14
Selection-amplification finds new RNA enzymes (ribozymes) among randomized RNAs with flanking unvaried sequences (primer complements). Precise removal of 3'-primer before reaction selected aminoacylation from PheAMP in three cycles, yielding active RNAs (k(cat) = 12-20 min(-1)) using only three conserved nucleotides, acting independently of divalent ions. This unusually simple RNA active site encouraged study of the reaction via molecular mechanics-based free energy minimization. On this basis, ...[more]