Expression artifact with retroviral vectors based on pBMN.
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ABSTRACT:
SUBMITTER: Fukunaga Y
PROVIDER: S-EPMC2753488 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Publications
Expression artifact with retroviral vectors based on pBMN.
Fukunaga Yoshitaka Y Svoboda Robert A RA Cerny Ronald L RL Johnson Keith R KR Wheelock Margaret J MJ
Analytical biochemistry 20090712 1
While characterizing various splice forms of p120 catenin, we observed what appeared to be a novel posttranslational modification of p120, resulting in a higher molecular weight form that was dependent on the splicing pattern. Further investigation revealed the higher molecular weight form to be a fusion protein between sequences encoded by the retroviral vector and p120. We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution ...[more]
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