ProMoST: a tool for calculating the pI and molecular mass of phosphorylated and modified proteins on two-dimensional gels.
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ABSTRACT:
SUBMITTER: Halligan BD
PROVIDER: S-EPMC2754287 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Publications
ProMoST: a tool for calculating the pI and molecular mass of phosphorylated and modified proteins on two-dimensional gels.
Halligan Brian D BD Halligan Brian D BD
Methods in molecular biology (Clifton, N.J.) 20090101
Protein modifications such as phosphorylation are often studied by two-dimensional gel electrophoresis, since the perturbation in the protein's pI value is readily detected by this method. It is important to be able to calculate the changes in the pI values that specific post-translational modifications cause and to visualize how these changes will effect protein migration on 2D gels. To address this need, we have developed ProMoST. ProMoST is a freely accessible Web-based application that calcu ...[more]
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