Quadruple 9-mer-based protein binding microarray with DsRed fusion protein.

ABSTRACT:

Background

The interaction between a transcription factor and DNA motif (cis-acting element) is an important regulatory step in gene regulation. Comprehensive genome-wide methods have been developed to characterize protein-DNA interactions. Recently, the universal protein binding microarray (PBM) was introduced to determine if a DNA motif interacts with proteins in a genome-wide manner.

Results

We facilitated the PBM technology using a DsRed fluorescent protein and a concatenated sequence of oligonucleotides. The PBM was designed in such a way that target probes were synthesized as quadruples of all possible 9-mer combinations, permitting unequivocal interpretation of the cis-acting elements. The complimentary DNA strands of the features were synthesized with a primer and DN

SUBMITTER: Kim MJ 

PROVIDER: S-EPMC2754467 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature