Ontology highlight
ABSTRACT:
SUBMITTER: Nishikawa J
PROVIDER: S-EPMC275550 | biostudies-literature | 2003 Nov
REPOSITORIES: biostudies-literature
Nishikawa Jun-ichi J Amano Miho M Fukue Yoshiro Y Tanaka Shigeo S Kishi Haruka H Hirota Yoshiko Y Yoda Kinya K Ohyama Takashi T
Nucleic acids research 20031101 22
There is little information on chromatin structure that allows access of trans-acting transcription factors. Logically, the target DNA elements become accessible by either exposing themselves towards the environment on the surface of the nucleosome, or making the regulatory region free of the nucleosome. Here, we demonstrate that curved DNA that mimics a negative supercoil can play both roles in the promoter region. By constructing 35 reporter plasmids and using in vivo assay systems, we scrutin ...[more]