Ontology highlight
ABSTRACT:
SUBMITTER: Pohjoismaki JL
PROVIDER: S-EPMC2755869 | biostudies-literature | 2009 Aug
REPOSITORIES: biostudies-literature
Pohjoismäki Jaakko L O JL Goffart Steffi S Tyynismaa Henna H Willcox Smaranda S Ide Tomomi T Kang Dongchon D Suomalainen Anu A Karhunen Pekka J PJ Griffith Jack D JD Holt Ian J IJ Jacobs Howard T HT
The Journal of biological chemistry 20090612 32
Analysis of human heart mitochondrial DNA (mtDNA) by electron microscopy and agarose gel electrophoresis revealed a complete absence of the -type replication intermediates seen abundantly in mtDNA from all other tissues. Instead only Y- and X-junctional forms were detected after restriction digestion. Uncut heart mtDNA was organized in tangled complexes of up to 20 or more genome equivalents, which could be resolved to genomic monomers, dimers, and linear fragments by treatment with the decatena ...[more]