Ontology highlight
ABSTRACT:
SUBMITTER: Camahort R
PROVIDER: S-EPMC2757638 | biostudies-literature | 2009 Sep
REPOSITORIES: biostudies-literature
Molecular cell 20090901 6
The budding yeast CenH3 histone variant Cse4 localizes to centromeric nucleosomes and is required for kinetochore assembly and chromosome segregation. The exact composition of centromeric Cse4-containing nucleosomes is a subject of debate. Using unbiased biochemical, cell-biological, and genetic approaches, we have tested the composition of Cse4-containing nucleosomes. Using micrococcal nuclease-treated chromatin, we find that Cse4 is associated with the histones H2A, H2B, and H4, but not H3 or ...[more]