Ontology highlight
ABSTRACT:
SUBMITTER: Daber R
PROVIDER: S-EPMC2760156 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Daber Robert R Sharp Kim K Lewis Mitchell M
Journal of molecular biology 20090722 5
In both prokaryotic and eukaryotic organisms, repressors and activators are responsible for regulating gene expression. The lac operon is a paradigm for understanding how metabolites function as signaling molecules and modulate transcription. These metabolites or allosteric effector molecules bind to the repressor and alter the conformational equilibrium between the induced and the repressed states. Here, we describe a set of experiments where we modified a single inducer binding site in a dimer ...[more]