Ontology highlight
ABSTRACT:
SUBMITTER: Wu P
PROVIDER: S-EPMC2764232 | biostudies-literature | 2003 Feb
REPOSITORIES: biostudies-literature
Wu Peiying P Qiu Chen C Sohail Anjum A Zhang Xing X Bhagwat Ashok S AS Cheng Xiaodong X
The Journal of biological chemistry 20021126 7
MBD4 is a member of the methyl-CpG-binding protein family. It contains two DNA binding domains, an amino-proximal methyl-CpG binding domain (MBD) and a C-terminal mismatch-specific glycosylase domain. Limited in vitro proteolysis of mouse MBD4 yields two stable fragments: a 139-residue fragment including the MBD, and the other 155-residue fragment including the glycosylase domain. Here we show that the latter fragment is active as a glycosylase on a DNA duplex containing a G:T mismatch within a ...[more]