High-potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa azurins.
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ABSTRACT:
SUBMITTER: Lancaster KM
PROVIDER: S-EPMC2765487 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
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High-potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa azurins.
Lancaster Kyle M KM Yokoyama Keiko K Richards John H JH Winkler Jay R JR Gray Harry B HB
Inorganic chemistry 20090201 4
Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated Cu(II/I) reduction potentials relative to the Cu(II) aquo ion. The high potential and low axial hyperfine splitting (Cu(II) electron paramagnetic resonance A( parallel)) of the C112D/M121L protein are remarkably similar to features normally associated with type 1 copper centers. ...[more]
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