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Accurate quantitation of standard peptides used for quantitative proteomics.

ABSTRACT: MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 microM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.

SUBMITTER: Bordeerat NK

PROVIDER: S-EPMC2766299 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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Accurate quantitation of standard peptides used for quantitative proteomics.

Bordeerat Narisa K NK Georgieva Nadia I NI Klapper David G DG Collins Leonard B LB Cross Tyra J TJ Borchers Christoph H CH Swenberg James A JA Boysen Gunnar G

Proteomics 20090801 15

MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 microM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the ...[more]

PMID: 19637239

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