Ontology highlight
ABSTRACT:
SUBMITTER: Mercer AC
PROVIDER: S-EPMC2767527 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Mercer Andrew C AC Meier Jordan L JL Torpey Justin W JW Burkart Michael D MD
Chembiochem : a European journal of chemical biology 20090401 6
Carrier proteins are central to the biosynthesis of primary and secondary metabolites in all organisms. Here we describe metabolic labeling and manipulation of native acyl carrier proteins in both type I and II fatty acid synthases. By utilizing natural promiscuity in the CoA biosynthetic pathway in combination with synthetic pantetheine analogues, we demonstrate metabolic labeling of endogenous carrier proteins with reporter tags in Gram-positive and Gram-negative bacteria and in a human carcin ...[more]