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Bipartite tetracysteine display requires site flexibility for ReAsH coordination.

ABSTRACT: Flexibility required: We designed intramolecular bipartite tetracysteine sites in loops of p53 and the beta-sheets of EmGFP. We found that ReAsH binding preferentially favors tetracysteine sites with flexible geometries such as loops; flexibility was assessed by comparing Calpha B-factor values. This information is important for directing successful bipartite tetracysteine site designs.

SUBMITTER: Goodman JL

PROVIDER: S-EPMC2773557 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Bipartite tetracysteine display requires site flexibility for ReAsH coordination.

Goodman Jessica L JL Fried Daniel B DB Schepartz Alanna A

Chembiochem : a European journal of chemical biology 20090701 10

Flexibility required: We designed intramolecular bipartite tetracysteine sites in loops of p53 and the beta-sheets of EmGFP. We found that ReAsH binding preferentially favors tetracysteine sites with flexible geometries such as loops; flexibility was assessed by comparing Calpha B-factor values. This information is important for directing successful bipartite tetracysteine site designs. ...[more]

PMID: 19533719

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