Ontology highlight
ABSTRACT:
SUBMITTER: Franzini RM
PROVIDER: S-EPMC2774910 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20091101 44
RNA-templated fluorescence activation is a nucleic acid detection strategy that offers the possibility of direct visual detection of genetic information in living cells. Here we describe a new reaction strategy for fluorescence activation in which a phosphine on one DNA probe reduces an azide group in a linker on a second probe, resulting in linker cleavage and release of a fluorescence quenching group. These "Q-STAR" probes are shown to yield a strong fluorescence turn-on signal in approximatel ...[more]