Ontology highlight
ABSTRACT:
SUBMITTER: Gasecka A
PROVIDER: S-EPMC2776251 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Gasecka Alicja A Han Tsai-Jung TJ Favard Cyril C Cho Bong Rae BR Brasselet Sophie S
Biophysical journal 20091101 10
We present a polarimetric two-photon microscopy technique to quantitatively image the local static molecular orientational behavior in lipid and cell membranes. This approach, based on a tunable excitation polarization state complemented by a polarized readout, is easily implementable and does not require hypotheses on the molecular angular distribution such as its mean orientation, which is a main limitation in traditional fluorescence anisotropy measurements. The method is applied to the inves ...[more]