Unknown

Dataset Information

0

Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.


ABSTRACT: The intracellular protozoan Toxoplasma gondii is among the most widespread parasites. The broad host cell range of the parasite can be explained by carbohydrate microarray screening analyses that have demonstrated the ability of the T. gondii adhesive protein, TgMIC1, to bind to a wide spectrum of sialyl oligosaccharide ligands. Here, we investigate by further microarray analyses in a dose-response format the differential binding of TgMIC1 to 2-3- and 2-6-linked sialyl carbohydrates. Interestingly, two novel synthetic fluorinated analogs of 3'SiaLacNAc(1-4) and 3'SiaLacNAc(1-3) were identified as highly potent ligands. To understand the structural basis of the carbohydrate binding specificity of TgMIC1, we have determined the crystal structures of TgMIC1 micronemal adhesive repeat (MAR)-region (TgMIC1-MARR) in complex with five sialyl-N-acetyllactosamine analogs. These crystal structures have revealed a specific, water-mediated hydrogen bond network that accounts for the preferential binding of TgMIC1-MARR to arrayed 2-3-linked sialyl oligosaccharides and the high potency of the fluorinated analogs. Furthermore, we provide strong evidence for the first observation of a C--F...H--O hydrogen bond within a lectin-carbohydrate complex. Finally, detailed comparison with other oligosaccharide-protein complexes in the Protein Data Bank (PDB) reveals a new family of sialic-acid binding sites from lectins in parasites, bacteria, and viruses.

SUBMITTER: Garnett JA 

PROVIDER: S-EPMC2777368 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.

Garnett James A JA   Liu Yan Y   Leon Ester E   Allman Sarah A SA   Friedrich Nikolas N   Saouros Savvas S   Curry Stephen S   Soldati-Favre Dominique D   Davis Benjamin G BG   Feizi Ten T   Matthews Stephen S  

Protein science : a publication of the Protein Society 20090901 9


The intracellular protozoan Toxoplasma gondii is among the most widespread parasites. The broad host cell range of the parasite can be explained by carbohydrate microarray screening analyses that have demonstrated the ability of the T. gondii adhesive protein, TgMIC1, to bind to a wide spectrum of sialyl oligosaccharide ligands. Here, we investigate by further microarray analyses in a dose-response format the differential binding of TgMIC1 to 2-3- and 2-6-linked sialyl carbohydrates. Interesting  ...[more]

Similar Datasets

| S-EPMC2982875 | biostudies-literature
| S-EPMC4509771 | biostudies-literature
| S-EPMC6082678 | biostudies-literature
| S-EPMC4850294 | biostudies-literature
| S-EPMC5391012 | biostudies-literature
| S-EPMC10471735 | biostudies-literature
| S-EPMC4187870 | biostudies-other
| S-EPMC2292621 | biostudies-literature
| S-EPMC1220475 | biostudies-other
| S-EPMC125952 | biostudies-literature