Ontology highlight
ABSTRACT:
SUBMITTER: Neely RK
PROVIDER: S-EPMC2777440 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Neely Robert K RK Tamulaitis Gintautas G Chen Kai K Kubala Marta M Siksnys Virginijus V Jones Anita C AC
Nucleic acids research 20090908 20
Restriction enzymes Ecl18kI, PspGI and EcoRII-C, specific for interrupted 5-bp target sequences, flip the central base pair of these sequences into their protein pockets to facilitate sequence recognition and adjust the DNA cleavage pattern. We have used time-resolved fluorescence spectroscopy of 2-aminopurine-labelled DNA in complex with each of these enzymes in solution to explore the nucleotide flipping mechanism and to obtain a detailed picture of the molecular environment of the extrahelica ...[more]