Ontology highlight
ABSTRACT:
SUBMITTER: Bagby SC
PROVIDER: S-EPMC2779684 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Bagby Sarah C SC Bergman Nicholas H NH Shechner David M DM Yen Catherine C Bartel David P DP
RNA (New York, N.Y.) 20091201 12
The class I ligase was among the first ribozymes to have been isolated from random sequences and represents the catalytic core of several RNA-directed RNA polymerase ribozymes. The ligase is also notable for its catalytic efficiency and structural complexity. Here, we report an improved version of this ribozyme, arising from selection that targeted the kinetics of the chemical step. Compared with the parent ribozyme, the improved ligase achieves a modest increase in rate enhancement under the se ...[more]