A replication clock for Mycobacterium tuberculosis.
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ABSTRACT:
SUBMITTER: Gill WP
PROVIDER: S-EPMC2779834 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Publications
A replication clock for Mycobacterium tuberculosis.
Gill Wendy P WP Harik Nada S NS Whiddon Molly R MR Liao Reiling P RP Mittler John E JE Sherman David R DR
Nature medicine 20090201 2
Few tools exist to assess replication of chronic pathogens during infection. This has been a considerable barrier to understanding latent tuberculosis, and efforts to develop new therapies generally assume that the bacteria are very slowly replicating or nonreplicating during latency. To monitor Mycobacterium tuberculosis replication within hosts, we exploit an unstable plasmid that is lost at a steady, quantifiable rate from dividing cells in the absence of antibiotic selection. By applying a m ...[more]
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