Unknown

Dataset Information

0

PGRL1 participates in iron-induced remodeling of the photosynthetic apparatus and in energy metabolism in Chlamydomonas reinhardtii.


ABSTRACT: PGRL1 RNA and protein levels are increased in iron-deficient Chlamydomonas reinhardtii cells. In an RNAi strain, which accumulates lower PGRL1 levels in both iron-replete and -starved conditions, the photosynthetic electron transfer rate is decreased, respiratory capacity in iron-sufficient conditions is increased, and the efficiency of cyclic electron transfer under iron-deprivation is diminished. Pgrl1-kd cells exhibit iron deficiency symptoms at higher iron concentrations than wild-type cells, although the cells are not more depleted in cellular iron relative to wild-type cells as measured by mass spectrometry. Thiol-trapping experiments indicate iron-dependent and redox-induced conformational changes in PGRL1 that may provide a link between iron metabolism and the partitioning of photosynthetic electron transfer between linear and cyclic flow. We propose, therefore, that PGRL1 in C. reinhardtii may possess a dual function in the chloroplast; that is, iron sensing and modulation of electron transfer.

SUBMITTER: Petroutsos D 

PROVIDER: S-EPMC2781694 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

PGRL1 participates in iron-induced remodeling of the photosynthetic apparatus and in energy metabolism in Chlamydomonas reinhardtii.

Petroutsos Dimitris D   Terauchi Aimee M AM   Busch Andreas A   Hirschmann Ingrid I   Merchant Sabeeha S SS   Finazzi Giovanni G   Hippler Michael M  

The Journal of biological chemistry 20090925 47


PGRL1 RNA and protein levels are increased in iron-deficient Chlamydomonas reinhardtii cells. In an RNAi strain, which accumulates lower PGRL1 levels in both iron-replete and -starved conditions, the photosynthetic electron transfer rate is decreased, respiratory capacity in iron-sufficient conditions is increased, and the efficiency of cyclic electron transfer under iron-deprivation is diminished. Pgrl1-kd cells exhibit iron deficiency symptoms at higher iron concentrations than wild-type cells  ...[more]

Similar Datasets

| S-EPMC5683061 | biostudies-literature
| S-EPMC126744 | biostudies-literature
| S-EPMC8821104 | biostudies-literature
| S-EPMC5720477 | biostudies-literature
| S-EPMC409943 | biostudies-literature
| S-EPMC9110296 | biostudies-literature
| S-EPMC2628641 | biostudies-literature
| S-EPMC3444938 | biostudies-literature
| S-EPMC2757591 | biostudies-literature
| S-EPMC6125352 | biostudies-literature