Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia.

ABSTRACT:

Background

Hypoxia-inducible factor 1 (HIF-1) plays a key role in cellular adaptation to hypoxia. To better understand the determinants of HIF-1 binding and transactivation, we used ChIP-chip and gene expression profiling to define the relationship between the epigenetic landscape, sites of HIF-1 binding, and genes transactivated by hypoxia in two cell lines.

Results

We found that when cells were acutely subjected to hypoxia, HIF-1 preferentially bound to loci that were already transcriptionally active under normal growth conditions characterized by the presence of histone H3 lysine 4 methylation, the presence of RNA polymerase II, and basal production of mRNA. Cell type-specific differences in HIF-1 binding were largely attributable to differences in the basal gene expressi

SUBMITTER: Xia X 

PROVIDER: S-EPMC2784328 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature