Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts.
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ABSTRACT:
SUBMITTER: Levin JZ
PROVIDER: S-EPMC2784330 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Publications
Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts.
Levin Joshua Z JZ Berger Michael F MF Adiconis Xian X Rogov Peter P Melnikov Alexandre A Fennell Timothy T Nusbaum Chad C Garraway Levi A LA Gnirke Andreas A
Genome biology 20091016 10
Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of " ...[more]
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