Ontology highlight
ABSTRACT:
SUBMITTER: Chamberland A
PROVIDER: S-EPMC2785663 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Chamberland Angela A Wang Eunice E Jones Aled R AR Collins-Racie Lisa A LA LaVallie Edward R ER Huang Ying Y Liu Lin L Morris Elisabeth A EA Flannery Carl R CR Yang Zhiyong Z
The Journal of biological chemistry 20090805 40
Mass spectrometry-based proteomic analyses performed on cartilage tissue extracts identified the serine protease HtrA1/PRSS11 as a major protein component of human articular cartilage, with elevated levels occurring in association with osteoarthritis. Overexpression of a catalytically active form of HtrA1, but not an active site mutant (S328A), caused a marked reduction in proteoglycan content in chondrocyte-seeded alginate cultures. Aggrecan degradation fragments were detected in conditioned me ...[more]