Development and application of quantitative-PCR tools for subgroups of the Roseobacter clade.
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ABSTRACT:
SUBMITTER: Buchan A
PROVIDER: S-EPMC2786420 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Publications
Development and application of quantitative-PCR tools for subgroups of the Roseobacter clade.
Buchan Alison A Hadden Mary M Suzuki Marcelino T MT
Applied and environmental microbiology 20091002 23
Specific SYBR green-based quantitative-PCR assays targeting conserved regions in the 16S-23S rRNA internal transcribed spacer regions were developed for five subgroups of the environmentally abundant and biogeochemically active Roseobacter clade of marine bacteria. The assays were applied to field samples demonstrating their utility in investigations of abundant Roseobacter group phylotypes in the environment. ...[more]
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