CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs.
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ABSTRACT:
SUBMITTER: Castanotto D
PROVIDER: S-EPMC2787469 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Publications
CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs.
Castanotto Daniela D Lingeman Robert R Riggs Arthur D AD Rossi John J JJ
Proceedings of the National Academy of Sciences of the United States of America 20091202 51
Drosha-processed microRNAs (miRNAs) have been shown to be exported from the nucleus to the cytoplasm by Exportin 5, where they are processed a second time to generate mature miRNAs. In this work we show that miRNAs also use CRM1 for nuclear-cytoplasmic shuttling. Inhibition of CRM1 by Leptomycin B results in nuclear accumulation of miRNA guide sequences. Nuclear to cytoplasmic transport can be actively competed by synthetic small interfering RNAs, indicating that this pathway is shared by differ ...[more]
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