Ciliogenesis in cryopreserved mammalian tracheal epithelial cells cultured at the air-liquid interface.

ABSTRACT: To determine air-liquid interface (ALI) culture derived from cryopreserved mammalian tracheal ciliated cells is a viable ciliated cell model for the investigations of regulatory mechanisms of ciliary beat frequency (CBF), two studies were performed using ovine and porcine tracheae obtained from local slaughterhouses. The protease-digested tracheal ciliated cells were harvested and cultured at the ALI using collagen-coated, porous membrane inserts. In study 1, the ALI culturing protocols were established using non-cryopreserved ovine tracheal ciliated cells. Ciliogenesis was documented with immuno-histology and electron micrographs. Vigorous beating cilia were video-recorded. CBF was measured by laser light scattering. The functional integrity of the autonomic receptors of the ciliated cell

SUBMITTER: Mao H 

PROVIDER: S-EPMC2787828 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature