Unknown

Dataset Information

0

Statistical methods for analysis of high-throughput RNA interference screens.


ABSTRACT: RNA interference (RNAi) has become a powerful technique for reverse genetics and drug discovery, and in both of these areas large-scale high-throughput RNAi screens are commonly performed. The statistical techniques used to analyze these screens are frequently borrowed directly from small-molecule screening; however, small-molecule and RNAi data characteristics differ in meaningful ways. We examine the similarities and differences between RNAi and small-molecule screens, highlighting particular characteristics of RNAi screen data that must be addressed during analysis. Additionally, we provide guidance on selection of analysis techniques in the context of a sample workflow.

SUBMITTER: Birmingham A 

PROVIDER: S-EPMC2789971 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications


RNA interference (RNAi) has become a powerful technique for reverse genetics and drug discovery, and in both of these areas large-scale high-throughput RNAi screens are commonly performed. The statistical techniques used to analyze these screens are frequently borrowed directly from small-molecule screening; however, small-molecule and RNAi data characteristics differ in meaningful ways. We examine the similarities and differences between RNAi and small-molecule screens, highlighting particular  ...[more]

Similar Datasets

| S-EPMC4597449 | biostudies-literature
| S-EPMC5001608 | biostudies-literature
| S-EPMC4577452 | biostudies-literature
| S-EPMC7216119 | biostudies-literature
| S-EPMC3226368 | biostudies-literature
| S-EPMC2811738 | biostudies-other
| S-EPMC6331134 | biostudies-literature
| S-EPMC5524642 | biostudies-literature
| S-EPMC4491794 | biostudies-other
| S-EPMC3333774 | biostudies-literature