Ontology highlight
ABSTRACT:
SUBMITTER: Zhang L
PROVIDER: S-EPMC2790073 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Zhang Liyun L Zheng Yuchuan Y Xi Zhaoyong Z Luo Zhaofeng Z Xu Xiaolong X Wang Chunyu C Liu Yangzhong Y
Molecular bioSystems 20090421 6
Zinc has been found in the crystal structures of inteins and the zinc ion can inhibit intein splicing both in vitro and in vivo. The interactions between metal ions and three minimized recA inteins have been studied in this work. Isothermal titration calorimetry (ITC) results show that the zinc binding affinity to three inteins is in the order of DeltaI-SM > DeltaDeltaI(hh)-SM approximately DeltaDeltaI(hh)-CM, but is much weaker than to EDTA. These data explain the reversible inhibition and the ...[more]