Ontology highlight
ABSTRACT:
SUBMITTER: Lamboy JA
PROVIDER: S-EPMC2790074 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Lamboy Jorge A JA Tam Phillip Y PY Lee Lucie S LS Jackson Pilgrim J PJ Avrantinis Sara K SK Lee Hye J HJ Corn Robert M RM Weiss Gregory A GA
Chembiochem : a European journal of chemical biology 20081101 17
An Achilles heel inherent to all molecular display formats, background binding between target and display system introduces false positives into screens and selections. For example, the negatively charged surfaces of phage, mRNA, and ribosome display systems bind with unacceptably high nonspecificity to positively charged target molecules, which represent an estimated 35% of proteins in the human proteome. Here we report the first systematic attempt to understand why a broad class of molecular d ...[more]