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Direct measurement of oxidative metabolism in the living brain by microdialysis: a review.

ABSTRACT: This review summarizes microdialysis studies that address the question of which compounds serve as energy sources in the brain. Microdialysis was used to introduce 14C-labeled glucose, lactate, pyruvate, glutamate, glutamine, and acetate into the interstitial fluid of the brain to observe their metabolism to 14CO2. Although glucose uptake from the systemic system supplies the carbon source for these compounds, compounds synthesized from glucose by the brain are subject to recycling including complete metabolism to CO2. Therefore, the brain utilizes multiple compounds in its domain to provide the energy needed to fulfill its function. The physiological conditions controlling metabolism and the contribution of compartmentation into different brain regions, cell types, and subcellular spaces are still unresolved. The aconitase inhibitor fluorocitrate, with a lower inhibition threshold in glial cells, was used to identify the proportion of lactate and glucose that was oxidized in glial cells versus neurons. The fluorocitrate data suggest that glial and neuronal cells are capable of utilizing both lactate and glucose for energy metabolism.

SUBMITTER: Zielke HR

PROVIDER: S-EPMC2792919 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

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Direct measurement of oxidative metabolism in the living brain by microdialysis: a review.

Zielke H Ronald HR Zielke Carol L CL Baab Peter J PJ

Journal of neurochemistry 20090501

This review summarizes microdialysis studies that address the question of which compounds serve as energy sources in the brain. Microdialysis was used to introduce 14C-labeled glucose, lactate, pyruvate, glutamate, glutamine, and acetate into the interstitial fluid of the brain to observe their metabolism to 14CO2. Although glucose uptake from the systemic system supplies the carbon source for these compounds, compounds synthesized from glucose by the brain are subject to recycling including com ...[more]

PMID: 19393005

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Project description:ObjectivesPreservation of brain health is an urgent priority for the world's ageing population. The evidence base for brain health optimisation strategies is rapidly expanding, but clear recommendations have been limited by heterogeneity in measurement of brain health outcomes. We performed a scoping review to systematically evaluate brain health measurement in the scientific literature to date, informing development of a core outcome set.DesignScoping review.Data sourcesMedline, APA PsycArticles and Embase were searched through until 25 January 2023.Eligibility criteria for selecting studiesStudies were included if they described brain health evaluation methods in sufficient detail in human adults and were in English language.Data extraction and synthesisTwo reviewers independently screened titles, abstracts and full texts for inclusion and extracted data using Covidence software.ResultsFrom 6987 articles identified by the search, 727 studies met inclusion criteria. Study publication increased by 22 times in the last decade. Cohort study was the most common study design (n=609, 84%). 479 unique methods of measuring brain health were identified, comprising imaging, cognitive, mental health, biological and clinical categories. Seven of the top 10 most frequently used brain health measurement methods were imaging based, including structural imaging of grey matter and hippocampal volumes and white matter hyperintensities. Cognitive tests such as the trail making test accounted for 286 (59.7%) of all brain health measurement methods.ConclusionsThe scientific literature surrounding brain health has increased exponentially, yet measurement methods are highly heterogeneous across studies which may explain the lack of clinical translation. Future studies should aim to develop a selected group of measures that should be included in all brain health studies to aid interstudy comparison (core outcome set), and broaden from the current focus on neuroimaging outcomes to include a range of outcomes.

Dataset Information

Direct measurement of oxidative metabolism in the living brain by microdialysis: a review.

Publications

Direct measurement of oxidative metabolism in the living brain by microdialysis: a review.

Similar Datasets

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