Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart.
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ABSTRACT:
SUBMITTER: Bohl S
PROVIDER: S-EPMC2793132 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Publications
Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart.
Bohl Steffen S Medway Debra J DJ Schulz-Menger Jeanette J Schneider Jurgen E JE Neubauer Stefan S Lygate Craig A CA
American journal of physiology. Heart and circulatory physiology 20091009 6
Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological analysis, however, remains technically challenging, and the resulting quality is often unsatisfactory. For this report we have scrutinized each step involved in standard double-staining histology. We have ...[more]
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