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ABSTRACT:
SUBMITTER: Dyer JH
PROVIDER: S-EPMC2793306 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Dyer James H JH Maina Anthony A Gomez Iris D ID Cadet Melissa M Oeljeklaus Silke S Schiedel Anke C AC
International journal of biological sciences 20091202 7
Thiolase I and II coexist as part of the glyoxysomal beta-oxidation system in sunflower (Helianthus annuus L.) cotyledons, the only system shown to have both forms. The importance of thiolases can be underscored not only by their ubiquity, but also by their involvement in a wide variety of processes in plants, animals and bacteria. Here we describe the cloning, expression and purification of acetoacetyl CoA thiolase (AACT) in enzymatically active form. Use of the extensive amount of sequence inf ...[more]