Ontology highlight
ABSTRACT:
SUBMITTER: MacLellan SR
PROVIDER: S-EPMC2795558 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
MacLellan Shawn R SR Guariglia-Oropeza Veronica V Gaballa Ahmed A Helmann John D JD
Proceedings of the National Academy of Sciences of the United States of America 20091125 50
The sigma-like factor YvrI and coregulator YvrHa activate transcription from a small set of conserved promoters in Bacillus subtilis. We report here that these two proteins independently contribute sigma-region 2 and sigma-region 4 functions to a holoenzyme-promoter DNA complex. YvrI binds RNA polymerase (RNAP) through a region 4 interaction with the beta-subunit flap domain and mediates specific promoter recognition but cannot initiate DNA melting at the -10 promoter element. Conversely, YvrHa ...[more]