Ontology highlight
ABSTRACT:
SUBMITTER: Muller-Steinhardt M
PROVIDER: S-EPMC2797812 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Müller-Steinhardt Michael M Schulte Friederike F Klüter Harald H Bugert Peter P
BMC research notes 20091210
<h4>Background</h4>Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G-->A), -572(G-->C) and -174(G-->C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 -597/-572/-174haplotyping are elaborate and require large amounts of genomic DNA.<h4>Findings</h4>We describe an improved typing technique requiring a decreased number of PCR-reactions and a reduced PCR-runtime due to optimized PCR-conditions.<h4>Conclusion</h4>This enables a fa ...[more]