Monitoring the dissemination of the broad-host-range plasmid pB10 in sediment microcosms by quantitative PCR.
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ABSTRACT:
SUBMITTER: Bonot S
PROVIDER: S-EPMC2798623 | biostudies-literature | 2010 Jan
REPOSITORIES: biostudies-literature
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Monitoring the dissemination of the broad-host-range plasmid pB10 in sediment microcosms by quantitative PCR.
Bonot Sébastien S Merlin Christophe C
Applied and environmental microbiology 20091106 1
Studying the transfer of specific mobile genetic elements in complex environmental matrices remains difficult because suitable molecular tools are not yet available to back up classical culture-dependent approaches. In this report, we show that quantitative PCR could be used to monitor the dissemination of the broad-host-range plasmid pB10 in sediment microcosms. This approach lies in the differential measurement of the host and plasmid DNAs used to inoculate the microcosms, using a particular d ...[more]
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