Nickel-inducible lysis system in Synechocystis sp. PCC 6803.
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ABSTRACT:
SUBMITTER: Liu X
PROVIDER: S-EPMC2799798 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Publications
Nickel-inducible lysis system in Synechocystis sp. PCC 6803.
Proceedings of the National Academy of Sciences of the United States of America 20091207 51
We designed and constructed a controllable inducing lysis system in Synechocystis sp. PCC 6803 to facilitate extracting lipids for biofuel production. Several bacteriophage-derived lysis genes were integrated into the genome and placed downstream of a nickel-inducible signal transduction system. We applied 3 strategies: (i) directly using the phage lysis cassette, (ii) constitutively expressing endolysin genes while restricting holin genes, and (iii) combining lysis genes from different phages. ...[more]
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