Ontology highlight
ABSTRACT:
SUBMITTER: Imker HJ
PROVIDER: S-EPMC2800832 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Imker Heidi J HJ Walsh Christopher T CT Wuest William M WM
Journal of the American Chemical Society 20091201 51
Syringolins are a class of cyclic tripeptide natural products that are potent and irreversible inhibitors of the eukaryotic proteasome. In addition to being hybrid NRPS/PKS molecules, they also feature an unusual ureido-linkage (red) between two amino acid monomers. Here we report the first in vitro characterization of enzymatic ureido-linkage formation which is catalyzed by an NRPS, SylC. Using (13)C- and (18)O-labeling studies, we show that biosynthesis occurs via N-carboxylation to form an in ...[more]