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Cre recombination-mediated cassette exchange for building versatile transgenic human embryonic stem cells lines.

ABSTRACT: To circumvent the silencing effect of transgene expression in human embryonic stem cells (hESCs), we employed the Cre recombination-mediated cassette exchange strategy to target the silencing-resistant site in the genome. We have identified new loci that sustain transgene expression during stem cell expansion and differentiation to cells representing the three germ layers in vitro and in vivo. The built-in double loxP cassette in the established master hESC lines was specifically replaced by a targeting vector containing the same loxP sites, using the cell-permeable Cre protein transduction method, resulting in successful generation of new hESC lines with constitutive functional gene expression, inducible transgene expression, and lineage-specific reporter gene expression. This strategy and the master cell lines allow for rapid production of transgenic hESC lines in ordinary laboratories.

SUBMITTER: Du ZW

PROVIDER: S-EPMC2801346 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

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Cre recombination-mediated cassette exchange for building versatile transgenic human embryonic stem cells lines.

Du Zhong-Wei ZW Hu Bao-Yang BY Ayala Melvin M Sauer Brian B Zhang Su-Chun SC

Stem cells (Dayton, Ohio) 20090501 5

To circumvent the silencing effect of transgene expression in human embryonic stem cells (hESCs), we employed the Cre recombination-mediated cassette exchange strategy to target the silencing-resistant site in the genome. We have identified new loci that sustain transgene expression during stem cell expansion and differentiation to cells representing the three germ layers in vitro and in vivo. The built-in double loxP cassette in the established master hESC lines was specifically replaced by a t ...[more]

PMID: 19415769

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