Ontology highlight
ABSTRACT:
SUBMITTER: Dlugosz M
PROVIDER: S-EPMC2802180 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
The journal of physical chemistry. B 20091101 47
We describe a method for determining diffusion-controlled rate constants for protein-protein association that explicitly includes the solution pH. The method combines the transient-complex theory for computing electrostatically enhanced association rates with an approach based on a rigorous thermodynamic cycle and partition functions for energy levels characterizing protonation states of associating proteins and their complexes. To test our method, we determine the pH-dependent kinetics of assoc ...[more]