Ontology highlight
ABSTRACT:
SUBMITTER: Lee HJ
PROVIDER: S-EPMC2802878 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Acta crystallographica. Section F, Structural biology and crystallization communications 20091127 Pt 12
Modification (HsdM) and specificity (HsdS) subunits are constituents of an active methyltransferase (MTase) of multifunctional type I restriction enzymes. To provide a molecular background on HsdM, a putative hsdM gene from Vibrio vulnificus YJ016 (HsdM_Vv) was cloned and the expressed protein was purified and crystallized from 22%(w/v) polyethylene glycol 8000, 0.02 M imidazole pH 7.5 and 5 mM beta-mercaptoethanol. Diffraction data were collected to 1.86 A resolution using synchrotron radiation ...[more]