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The Akt C-terminal modulator protein is an acyl-CoA thioesterase of the Hotdog-Fold family.

ABSTRACT: Herein, we report on an in vitro kinetic activity analysis that demonstrates that the protein known as the Akt C-terminal modulator protein is a broad-range, high-activity acyl-CoA thioesterase. In vitro tests of possible activity regulation by product inhibition or by Akt1 binding gave negative results. Truncation mutants confined the thioesterase activity to the C-terminal domain, consistent with our threading model. The N-terminal domain of unknown fold and function was found to contribute to solubility.

SUBMITTER: Zhao H 

PROVIDER: S-EPMC2803014 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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The Akt C-terminal modulator protein is an acyl-CoA thioesterase of the Hotdog-Fold family.

Zhao Hong H   Martin Brian M BM   Bisoffi Marco M   Dunaway-Mariano Debra D  

Biochemistry 20090601 24

Herein, we report on an in vitro kinetic activity analysis that demonstrates that the protein known as the Akt C-terminal modulator protein is a broad-range, high-activity acyl-CoA thioesterase. In vitro tests of possible activity regulation by product inhibition or by Akt1 binding gave negative results. Truncation mutants confined the thioesterase activity to the C-terminal domain, consistent with our threading model. The N-terminal domain of unknown fold and function was found to contribute to  ...[more]

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