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Feasibility of one-shot-per-crystal structure determination using Laue diffraction.

ABSTRACT: Crystal size is an important factor in determining the number of diffraction patterns which may be obtained from a protein crystal before severe radiation damage sets in. As crystal dimensions decrease this number is reduced, eventually falling to one, at which point a complete data set must be assembled using data from multiple crystals. When only a single exposure is to be collected from each crystal, the polychromatic Laue technique may be preferable to monochromatic methods owing to its simultaneous recording of a large number of fully recorded reflections per image. To assess the feasibility of solving structures using single Laue images from multiple crystals, data were collected using a 'pink' beam at the CHESS D1 station from groups of lysozyme crystals with dimensions of the order of 20-30 microm mounted on MicroMesh grids. Single-shot Laue data were used for structure determination by molecular replacement and correct solutions were obtained even when as few as five crystals were used.

SUBMITTER: Cornaby S

PROVIDER: S-EPMC2803125 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

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Feasibility of one-shot-per-crystal structure determination using Laue diffraction.

Cornaby Sterling S Szebenyi Doletha M E DM Smilgies Detlef-M DM Schuller David J DJ Gillilan Richard R Hao Quan Q Bilderback Donald H DH

Acta crystallographica. Section D, Biological crystallography 20091221 Pt 1

Crystal size is an important factor in determining the number of diffraction patterns which may be obtained from a protein crystal before severe radiation damage sets in. As crystal dimensions decrease this number is reduced, eventually falling to one, at which point a complete data set must be assembled using data from multiple crystals. When only a single exposure is to be collected from each crystal, the polychromatic Laue technique may be preferable to monochromatic methods owing to its simu ...[more]

PMID: 20057043

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Dataset Information

Feasibility of one-shot-per-crystal structure determination using Laue diffraction.

Publications

Feasibility of one-shot-per-crystal structure determination using Laue diffraction.

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