Characterization and physical mapping of ribosomal RNA gene families in Plantago.

ABSTRACT:

Background and aims

The organization of rRNA genes in cultivated Plantago ovata Forsk. and several of its wild allies was analysed to gain insight into the phylogenetic relationships of these species in the genus which includes some 200 species.

Methods

Specific primers were designed to amplify the internal transcribed spacer (ITS1 and ITS2) regions from seven Plantago species and the resulting fragments were cloned and sequenced. Similarly, using specific primers, the 5S rRNA genes from these species were amplified and subsequently cloned. Fluorescence in-situ hybridization (FISH) was used for physical mapping of 5S and 45S ribosomal RNA genes.

Results

The ITS1 region is 19-29 bp longer than the ITS2 in different Plantago species. The 5S rRNA gene-repeating unit vari

SUBMITTER: Dhar MK 

PROVIDER: S-EPMC2803661 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature