Dataset Information

Tissue distibution of murine Muc19/smgc gene products.

ABSTRACT: The recently identified gene Muc19/Smgc encodes two diverse splice variants, Smgc (submandibular gland protein C) and Muc19 (mucin 19). Muc19 is a member of the large gel-forming mucin family and is an exocrine product of sublingual mucous salivary glands in mice. SMGC is a transiently expressed secretion product of developing rodent submandibular and sublingual glands. Little is known about the expression of Muc19/Smgc gene products in other murine salivary and non-salivary tissues containing the mucous cell phenotype. Muc19 expression was therefore initially assessed by RT-PCR and immunohistochemistry. As a complementary approach, we developed a knockin mouse model, Muc19-EGFP, in which mice express a fusion protein containing the first 69 residues of Muc19 followed by enhanced green fluorescent protein (EGFP) as a marker of Muc19 expression. Results from both approaches are consistent, with preferential Muc19 expression in salivary major and minor mucous glands as well as submucosal glands of the tracheolarynx and bulbourethral glands. Evidence also indicates that individual mucous cells of minor salivary and bulbourethral glands produce another gel-forming mucin in addition to Muc19. We further find tissue expression of full-length Smgc transcripts, which encode for SMGC, and are restricted to neonatal tracheolarynx and all salivary tissues.

SUBMITTER: Das B

PROVIDER: S-EPMC2803704 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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Tissue distibution of murine Muc19/smgc gene products.

Das Biswadip B Cash Melanie N MN Hand Arthur R AR Shivazad Armin A Grieshaber Scott S SS Robinson Bently B Culp David J DJ

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 20100201 2

The recently identified gene Muc19/Smgc encodes two diverse splice variants, Smgc (submandibular gland protein C) and Muc19 (mucin 19). Muc19 is a member of the large gel-forming mucin family and is an exocrine product of sublingual mucous salivary glands in mice. SMGC is a transiently expressed secretion product of developing rodent submandibular and sublingual glands. Little is known about the expression of Muc19/Smgc gene products in other murine salivary and non-salivary tissues containing t ...[more]

PMID: 19826070

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Dataset Information

Tissue distibution of murine Muc19/smgc gene products.

Publications

Tissue distibution of murine Muc19/smgc gene products.

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