The ClC-3 Cl-/H+ antiporter becomes uncoupled at low extracellular pH.
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ABSTRACT:
SUBMITTER: Matsuda JJ
PROVIDER: S-EPMC2807314 | biostudies-literature | 2010 Jan
REPOSITORIES: biostudies-literature
Publications
The ClC-3 Cl-/H+ antiporter becomes uncoupled at low extracellular pH.
Matsuda James J JJ Filali Mohammed S MS Collins Malia M MM Volk Kenneth A KA Lamb Fred S FS
The Journal of biological chemistry 20091119 4
Adenovirus expressing ClC-3 (Ad-ClC-3) induces Cl(-)/H(+) antiport current (I(ClC-3)) in HEK293 cells. The outward rectification and time dependence of I(ClC-3) closely resemble an endogenous HEK293 cell acid-activated Cl(-) current (ICl(acid)) seen at extracellular pH <or= 5.5. ICl(acid) was present in smooth muscle cells from wild-type but not ClC-3 null mice. We therefore sought to determine whether these currents were related. ICl(acid) was larger in cells expressing Ad-ClC-3. Protons shifte ...[more]
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