Identification of differential gene expression in Brassica rapa nectaries through expressed sequence tag analysis.

ABSTRACT:

Background

Nectaries are the floral organs responsible for the synthesis and secretion of nectar. Despite their central roles in pollination biology, very little is understood about the molecular mechanisms underlying nectar production. This project was undertaken to identify genes potentially involved in mediating nectary form and function in Brassica rapa.

Methodology and principal findings

Four cDNA libraries were created using RNA isolated from the median and lateral nectaries of B. rapa flowers, with one normalized and one non-normalized library being generated from each tissue. Approximately 3,000 clones from each library were randomly sequenced from the 5' end to generate a total of 11,101 high quality expressed sequence tags (ESTs). Sequence assembly of all ESTs toge

SUBMITTER: Hampton M 

PROVIDER: S-EPMC2808342 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature