Unknown

Dataset Information

0

Immature small ribosomal subunits can engage in translation initiation in Saccharomyces cerevisiae.


ABSTRACT: It is generally assumed that, in Saccharomyces cerevisiae, immature 40S ribosomal subunits are not competent for translation initiation. Here, we show by different approaches that, in wild-type conditions, a portion of pre-40S particles (pre-SSU) associate with translating ribosomal complexes. When cytoplasmic 20S pre-rRNA processing is impaired, as in Rio1p- or Nob1p-depleted cells, a large part of pre-SSUs is associated with translating ribosomes complexes. Loading of pre-40S particles onto mRNAs presumably uses the canonical pathway as translation-initiation factors interact with 20S pre-rRNA. However, translation initiation is not required for 40S ribosomal subunit maturation. We also provide evidence suggesting that cytoplasmic 20S pre-rRNAs that associate with translating complexes are turned over by the no go decay (NGD) pathway, a process known to degrade mRNAs on which ribosomes are stalled. We propose that the cytoplasmic fate of 20S pre-rRNA is determined by the balance between pre-SSU processing kinetics and sensing of ribosome-like particles loaded onto mRNAs by the NGD machinery, which acts as an ultimate ribosome quality check point.

SUBMITTER: Soudet J 

PROVIDER: S-EPMC2808361 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Immature small ribosomal subunits can engage in translation initiation in Saccharomyces cerevisiae.

Soudet Julien J   Gélugne Jean-Paul JP   Belhabich-Baumas Kamila K   Caizergues-Ferrer Michèle M   Mougin Annie A  

The EMBO journal 20091105 1


It is generally assumed that, in Saccharomyces cerevisiae, immature 40S ribosomal subunits are not competent for translation initiation. Here, we show by different approaches that, in wild-type conditions, a portion of pre-40S particles (pre-SSU) associate with translating ribosomal complexes. When cytoplasmic 20S pre-rRNA processing is impaired, as in Rio1p- or Nob1p-depleted cells, a large part of pre-SSUs is associated with translating ribosomes complexes. Loading of pre-40S particles onto mR  ...[more]

Similar Datasets

| S-EPMC4615593 | biostudies-literature
| S-EPMC5961077 | biostudies-literature
| S-EPMC1840066 | biostudies-literature
| S-EPMC1370453 | biostudies-literature
| S-EPMC3081013 | biostudies-literature
| S-EPMC4279022 | biostudies-literature
| S-EPMC3884665 | biostudies-literature
| S-EPMC1382306 | biostudies-literature
| S-EPMC4750496 | biostudies-literature
| S-EPMC3209790 | biostudies-literature