Creating a novel origin of replication through modulating DNA-protein interfaces.

ABSTRACT:

Background

While the molecular mechanisms of DNA-protein specificity at the origin of replication have been determined in many model organisms, these interactions remain unknown in the majority of higher eukaryotes and numerous vertebrate viruses. Similar to many viral origins of replication, adeno-associated virus (AAV) utilizes a cis-acting origin of replication and a virus specific Replication protein (Rep) to faithfully carry out self-priming replication. The mechanisms of AAV DNA replication are generally well understood. However, the molecular basis of specificity between the Rep protein and the viral origin of replication between different AAV serotypes remains uncharacterized.

Methodology/principal findings

By generating a panel of chimeric and mutant origins between

SUBMITTER: Hewitt FC 

PROVIDER: S-EPMC2809752 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature