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TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins.

ABSTRACT: A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of (15)N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232-13237, 2007), by preserving the TROSY effect during the mixing period as well as the frequency encoding periods.

SUBMITTER: Li Y

PROVIDER: S-EPMC2810196 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature

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TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins.

Li Ying Y Palmer Arthur G AG

Journal of biomolecular NMR 20091105 4

A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of (15)N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232-13237, 2007), by preserving the TROSY effect during the mixing period as well as ...[more]

PMID: 19890725

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